McClung's Jenner-Giemsa
Malaria Parasites & General Oversight

Solutions

Stock Giemsa

Stock Jenner

Working Jenner
Stock Jenner	1	volume
Distilled water	1	volume
Make the diluted solution just before using.

Working Giemsa
Stock Giemsa	1	volume
Distilled water	10	volumes
Make the diluted solution just before using.

Dehydration fluids
Acetone	95	volumes,	Xylene	5	volumes	(5% xylene in acetone)
Acetone	70	volumes,	Xylene	30	volumes	(30% xylene in acetone)
Acetone	50	volumes,	Xylene	50	volumes	(Equal parts xylene and acetone)
Acetone	30	volumes,	Xylene	70	volumes	(30% acetone in xylene)
Acetone	5	volumes,	Xylene	95	volumes	(5% acetone in xylene)

Tissue sample
A fixative containing mercuric chloride and ethanol is recommended, but many fixatives may be used, although staining results may vary. 3µ paraffin sections of neutral buffered formalin fixed tissue are usually suitable.

Method

1. Bring sections to water with xylene and ethanol.
2. Remove mercury pigment if necessary.
3. Rinse well with distilled water.
4. Place into working Jenner for 4 minutes.
5. Without rinsing, place into working Giemsa 15-20 minutes.
6. Rinse well with distilled water to differentiate.
7. Dehydrate with the dehydratiion fluids in the order given above.
8. Clear with xylene and mount with a synthetic resinous medium.

Expected results

• Nuclei of tissue – blue-purple blue
• Cytoplasm of tissue – pink
• Nuclei of parasite – red-purple red
• Cytoplasm of parasite – blue
• Schüffner's stippling – red

Notes

1. If the sections are too blue, dilute acetic acid in distilled water may be used to remove the excess (1% or less). Wash with distilled water afterwards to remove residual acid.

 

Reference
Humason, G.L., (1967)
Animal Tissue Techniques
W.H. Freeman & Co., San Francisco, CA, USA

Translate in Google Translate Instructions