McNamara's Giemsa
General Oversight stain

Solutions

Stock Giemsa

Working Giemsa
Stock Giemsa	10	mL
Acetone	10	mL
Methanol	10	mL
Distilled water	100	mL
Make the diluted solution just before using.

Colophonium solution
Colophonium	15	g
Absolute ethanol	100	mL

Rinse solution
Acetone	70	mL
Xylene	30	mL

Tissue sample
Helly or neutral buffered formalin fixed tissue are recommended. Thin (3µ) sections are desirable.

Method

1. Bring sections to water with xylene and ethanol.
2. Rinse well with distilled water.
3. Place into working Giemsa for 10 to 15 minutes.
4. Rinse with tap water water and check staining microscopically.
5. Differentiate with colophonium solution until colour contrast is satisfactory.
   Control microscopically. If a film develops renew the colophonium solution.
6. Rinse several times with rinse solution. Do not allow to dry.
7. Rinse several times with xylene. Do not allow to dry.
8. Mount with a synthetic resinous medium.

Expected results

• Nuclei  –  blue-purple
• Cytoplasm  –  pink

 

Reference
Putt, F.A., (1972)
Manual of histopathological staining methods
John Wiley & Sons, New York, NY, USA

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