Gallego's Carbol Fuchsin
for Nuclei

Solutions

Solution A
Carbol fuchsin	2	mL
Distilled water	98	mL

Solution B
Formalin, conc. (40%)	1	mL
Distilled water	99	mL

Tissue sample
5µ paraffin sections of neutral buffered formalin fixed tissue are suitable. Other fixatives are likely to be satisfactory.

Method

1. Bring sections to water via xylene and ethanol.
2. Place into solution A for 5 minute.
3. Wash with water.
4. Place into solution B for 5 minutes.
5. Wash with water.
6. Stain with a contrast stain.
7. Dehydrate with ethanol.
8. Clear with xylene and mount with a resinous medium.

Expected results

• Nuclei  –  blue-black
• Cytoplasm  –  as counterstain

Reference
Gray, Peter. (1954)
The Microtomist's Formulary and Guide.
Originally published by:– The Blakiston Co.
Republished by:– Robert E. Krieger Publishing Co.
  Citing:–
    Gallego, (1919)
    Trabajos del Laboratorio de investigaciones biológicas de la Universidad de Madrid,
    v. 17, pp.95
  And:–
    Biot, (1910)
    Compte rendu de l'Association des anatomistes, Congrès de Lyon, pp.234

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