Toluidine Blue
for Helicobacter

Solutions

Stock toluidine blue
Toluidine blue	0.1	g
Phosphate buffer, pH 6.8	100	mL

Working toluidine blue
Stock toluidine blue	1	part
Tap water	9	parts

Tissue sample
5µ paraffin sections of formalin fixed tissue are suitable.

Method

1. Bring sections to water via xylene and ethanol.
2. Rinse with distilled water.
3. Place in working toluidine blue for 10 minutes.
4. Rinse with distilled water.
5. Dehydrate, clear and mount with a resinous medium.

Expected results

• Helicobacter  –  dark blue
• Nuclei  –  dark blue
• Background  –  light blue

Notes

1. Thin sections show the organisms best.
2. Adjust the working dilution and staining time for best results.
3. Hematology buffer tablets are suitable for making the stock solution.

 

Reference
A standard procedure with no specific reference.

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