Lillie's Allochrome

Solutions

1% Aqueous periodic acid

Schiff's reagent

Weigert's iron hematoxylin

Picro-methyl blue
Saturated aqueous picric acid	100	mL
1% aqueous methyl blue	0.4	mL

Tissue sample
5 µ paraffin sections. Most fixatives, including 10% NBF, are satisfactory but those generally considered preferable for trichrome stains will be advantageous.

Method

1. Bring sections to water via xylene and ethanol
2. Do a PAS stain, but do not counterstain
3. Stain nuclei with Weigert's iron hematoxylin or equivalent.
4. Wash well in tap water, rinse with distilled water.
5. Place into picro-methyl blue for 6 minutes.
6. Rinse well with 95% ethanol.
7. Dehydrate with absolute ethanol.
8. Clear with xylene and mount with a resinous medium.

Expected results

• Nuclei – Brown-black
• Collagen & reticulin – blue
• Cytoplasm & muscle – yellow
• PAS positive structures – red

Reference
Histological demonstration techniques, (1974))
Cook, H C.
Butterworths, London, England

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