Hitchcock Ehrich
for Plasma Cells

This method uses malachite green and acridine red to stain plasma cells in a similar manner to the methyl green pyronin methods.

Solutions

Solution A
Malachite green	1	g
Distilled water	100	mL

Solution B
Acridine red	3	g
Distilled water	100	mL

Combine 1 part of solution A with 3 parts of solution B immediately before use.

Tissue sample
Zenker fixation is recommended. Other fixatives may not be satisfactory.

Method

1. Bring sections to water via xylene and ethanol.
2. Place into the staining solution for 30 seconds.
3. Rinse with water.
4. Dehydrate rapidly with absolute ethanol.
5. Clear with xylene and mount with a resinous medium.

Expected results

• Nuclei  –  green
• Plasma cell cytoplasm  –  crimson
• Other cell cytoplasm  –  pink

 

Reference
Biological Staining Methods, 6th ed. (1957)
Gurr George T.,
George T. Gurr, London, UK