Rossman's Fixative
| Picric acid, sat. ethanolic | 900 | mL |
| Formalin, concentrated | 100 | mL |
Rossman's fluid is similar to Gendre's fluid, which itself is often thought of as an alcoholic Bouin's fluid. Fixatives containing ethanol and picric acid are often recommended for carbohydrates, particularly glycogen, as both chemicals enhance the preservation of glycogen either by trapping it within precipitated protein or by precipitation directly.
Reference
Culling, C.F.A.,
Handbook of Histopathological and Histochemical Techniques, Ed. 3,
Butterworths, London, UK.
