Newcomer's Fluid
| iso-Propanol | 60 | mL |
| Propionic acid | 30 | mL |
| Petroleum ether | 10 | mL |
| Acetone | 10 | mL |
| Dioxane | 10 | mL |
Recommended for chromosomes and the Feulgen reaction for DNA. It is said to preserve mucopolysaccharides well.
Note that dioxane is different from dioxin. There is a Wikipedia article on dioxane and another on dioxin.
Since this mixture contains suspect carcinogens and ethers, treat with caution and use in a fume chamber.
Time
A few hours to overnight, depending on tissue thickness.
Aftertreatment
Transfer to absolute ethanol or clearant.
Reference
Culling, C.F.A.,
Handbook of Histopathological and Histochemical Techniques, Ed. 3,
Butterworths, London, UK.
