Methylene Blue

Property Data
Common name
Suggested name
Other names
C.I. number
C.I. name
 
Class
Ionisation
Solubility aqueous
Solubility ethanol
Absorption maximum
 
 
 
Colour
Empirical formula
Formula weight
Methylene blue
Methylene blue
Swiss blue
52015
Basic blue 9
Solvent blue 8
Thiazin
Basic
3.55%
1.48%
661 (Aldrich)
664-666 (Conn)
668, 609 (Merck)
665 (Gurr)
Blue
C16H18N3SCl
319.9

Methylene blue is a very commonly used dye. It stains nuclei blue, and is often used for that purpose. Following "ripening", a process involving atmospheric, or chemical, oxidation and the production of the three strongly metachromatic dyes Azure A, B and C. This solution is called polychrome methylene blue and is very valuable for staining rapid frozen sections. Due to it's strong metachromatic staining properties, it can be used for the demonstration of mucins, cartilage, mast cells, etc.. A comparison of these dyes shows the relationship clearly.

 

Reference
R. D. Lillie.
Conn's Biological Stains
Williams & Wilkins, Baltimore, MD., U.S.A.

Aldrich chemical catalogue, 1992
Aldrich Chemical Company, Milwaukee, WI, USA.

Edward Gurr, (1971)
Synthetic dyes in biology, medicine and chemistry
Academic Press, London, England.

Susan Budavari, Editor, (1996)
The Merck Index, Ed. 12
Merck & Co., Inc., Whitehouse Station, NJ, USA

 


 

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