Stainsfile - Amido black 10B

Property	Data
Common name Suggested name Other names C.I. number C.I. name Class Ionisation Solubility aqueous Solubility ethanol Absorption maximum Colour Empirical formula Formula weight	Amido black 10B Amido black 10B Amidoschwarz Naphthol blue black 20470 Acid black 1 Azo Acid Readily Readily 618 (Aldrich) 624 (Gurr) Blue black C₂₂H₁₄N₆O₉S₂Na₂ 616.516

In conjunction with picric acid, in a Van Gieson type procedure, this dye demonstrates collagen and reticulin.

It is chemically very similar to Napthalene blue black CS, a dye recommended by Lendrum for the demonstration of the oldest fibrin but which is now difficult to obtain. Amido black 10B may be suitable as a substitute.

Compare both dyes.

Reference
R. D. Lillie.
Conn's Biological Stains
Williams & Wilkins, Baltimore, MD., U.S.A.

Aldrich chemical catalogue, 1992
Aldrich Chemical Company, Milwaukee, WI, USA.

Edward Gurr, (1971)
Synthetic dyes in biology, medicine and chemistry
Academic Press, London, England.

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Last updated January 2010.